Showing posts with label PCR. Show all posts
Showing posts with label PCR. Show all posts

Wednesday, May 9, 2012

CSI: Collecting Evidence at a Homicide


Even those naive to the inner workings of a crime scene investigation know that the collection, preservation, and analysis of evidence can all be the difference between a conviction and an acquittal. Tantamount to this is the fact that all of these evidentiary factors can also mean the difference between convicting a guilty person and convicting an innocent person. Crime scene investigating is an amalgamation of science, logic, and law. Although physical evidence is only part of the equation—with the ultimate goal being to convict the guilty party(s) of the crime—it does deserve special attention and training in and of itself in order to ensure the evidence is effectively used to help solve the crime.

At this particular crime scene (hypothetical crime scene), the investigation involves four key pieces of physical evidence that must be accounted for: dried blood, a handgun, shell casings, and hairs. When collecting these pieces of evidence, the investigator must follow strict protocol to ensure the preservation of such evidence.

Dried Blood


Dried blood will show up at just about every crime scene involving murder. There are several protocols used by investigators to ensure that this evidence gets to the lab with as little harm done to it as possible. If the dried blood is on clothing, for instance, it should be wrapped in clean paper, placed in a brown paper bag or box, sealed and labeled; the investigator should not attempt to remove the stain(s) from the cloth. If, by chance, the dried blood is on a small solid objects; the investigator should send the whole stained object to the Laboratory, after labeling and packaging it.

If the blood happens to be on large solid objects; the investigator should cover the stained area with clean paper and seal the edges down with tape to prevent loss or contamination. If it is not practical to transport the entire object to the lab, the blood stain should be scraped onto a clean piece of paper that can be folded and placed in an envelope. It should not be scraped directly into evidence envelope; rather, it should be scraped from objects using a freshly washed and dried knife or similar tool. The investigator should take precaution by washing and drying the tool before each stain is scraped off, before sealing and marking the envelope. Dried blood stains should not be mixed, but rather they should each be placed in a separate envelope, and they should never be wiped from an object using a moist cloth or paper.

Using various testing methods at the lab, such as the restriction fragment length polymorphism (RFLP) process or the polymerase chain reaction (PCR) process, blood can be examined and amplified in order to determine the genetic profile of the individual who left it behind.

Handgun


Ballistic evidence can lend a lot to an investigation, answering questions such as what type of gun was used, how many shots were fired, and even where the shooter was positioned when firing in relation to the victim. The most obvious rule of collecting a gun from a crime scene is to never submit a loaded gun to the lab unless it is delivered in person. Unfired cartridges should be left in the magazine of a weapon, provided the magazine is removed from the gun—a firearm with the cartridge in the chamber should never be shipped by any method, even if the weapon is not cocked or on safety.

The serial number should be recorded as well as should be the make, model, and caliber of the weapon, and it should be marked in some inconspicuous manner so it does not detract from its value before sending it to the Laboratory. Weapons should be placed in strong cardboard or wooden boxes and be well packed to prevent shifting of guns in transit. Also, rifles or shotguns should not be taken apart on the scene; this should be left to ballistics experts. If blood or any other material, which may pertain to an investigation, is present on the gun, the gun should be wrapped in clean paper and sealed with tape to prevent movement of the gun and loss of the sample during shipment.

Investigators should never clean the bore, chamber, or cylinder before submitting a firearm, and never attempt to fire the gun before it is examined in the lab. A handgun should be picked up with gloved hands (with index finger and thumb) on an area of the weapon that is unlikely to produce useful fingerprints, such as by the curvature around the trigger. A submerged weapon should be sealed in a plastic container while still under water.

Shell Casings


Shell casings can tell investigators several things about how the crime took place. Shell casings can also tell investigators what caliber, brand, and model of the gun used, as well as if the marks on the casings may show that the gun is involved in other investigations. Casings should be wrapped and sealed in separate labeled pill boxes or envelopes.

Striation marks (or microscopic scratches) inside of the barrel of the gun transfer onto the bullets when they are fired. These marks are unique to each barrel, which allows forensic investigators to identify a weapon from the bullets fired from it. A lack of shell casings at a scene could mean that the shooting took place elsewhere, the weapon didn’t eject the spent shells, or the shooter took the time to clean them before leaving.

The gun can also be fired back at the lab by ballistic experts in order to determine if the bullets found at the scene came from the gun in question and how close the gun was fired from. Firearm and tool mark examinations (called “ballistic fingerprinting”) is also done by ballistic experts.

Hairs


Hair left at the crime scene can also be a valuable piece of evidence in an investigation. Along from the many things that it can reveal, it can sometimes reveal the possible race of the individual from whom it came and the part of the body from which it originated. All hair should be recovered from a scene. Hair should be collected using gloved fingers or tweezers, placed in paper bindles or coin envelopes, folded and sealed in larger envelopes, and then labeled on the outer sealed envelope.

If hair is attached, such as in dry blood, or caught in metal or a crack of glass, the investigator should not attempt to remove it, but should rather leave the hair together with the object. If the object is small, it should be marked, wrapped, and sealed into an envelope. If the object is large, the area containing the hair should be wrapped in paper to prevent loss of hairs during shipment. In the lab, human hair can be compared to determine whether or not two samples could have had a common origin.

Friday, August 14, 2009

DNA Fingerprinting Using the PCR Process


DNA Fingerprinting (a.k.a. DNA Profiling or DNA analysis) is a sub-category of Biotechnology that has several uses among scientists as well as other fields. A broad definition of Biotechnology is, “any use or alteration of organisms, cells, or biological molecules to achieve specific practical goals” (Audersirk, Audersirk, & Byers, 2007).

Deoxyribonucleic acid (DNA) is located in the nucleus of every cell that has a nucleus. Its appearance is similar to a twisted ladder or staircase, which is referred to as a double-helix. DNA is an extremely long polymer made from four nucleotides: Adenine (A), Guanine (G), Cytosine (C), and Thymine (T). It is the sequence of A, G, T, and C that codes information for each gene.

In 1986 Kary B. Mullis developed the Polymerase Chain Reaction process (PCR), that produces Short Tandem Repeats (STR) which are relatively small fragments of DNA (Audersirk, Audersirk, & Byers, 2007). This means that very small amounts of DNA, found at a crime scene for instance, can be multiplied by the PCR process.

There are two main reasons why the PCR process was such a huge breakthrough. The previous system took nearly four-five weeks for results to return from the lab, but PCR could return results within twenty-four hours (Ragle, 2002). Another reason was that the previous process required almost perfect samples of DNA, and there has to be a large amount to test successfully; while the PCR process requires a relatively small amount of DNA and is successful with almost every sample (Ragle, 2002).

Once in the lab, the DNA sample needs to be amplified. To do this, the DNA double-helix needs to be separated first. Heating a solution of the DNA to a temperature of 90C separates the two strands. After the strands unwind and cool, they are put into a DNA Amplifier and an enzyme called polymerase makes two new DNA strands; which are exact duplicates of the original. It takes approximately 4 minutes per cycle; each cycle doubling the amount of DNA. This process can be repeated every 4 minutes, which comes to 30 cycles every 2 hours. This means that in 2 hours, the small sample has been amplified 2^30 or 1 billion times.

“In 1999, British and American law enforcement agencies agreed to use a set of 10 to 13 STR’s…that vary greatly among individuals. A perfect match of 10 STR’s in a suspects DNA and DNA found at a crime scene means that there is less than one chance in a trillion that the two DNA samples did not come from the same person” (Audersirk, Audersirk, & Byers, 2007). In this can be realized the power and significance of this system.

In 1990 the FBI formed a “working group” to come up with a national data base that would hold all DNA Profiles collected (Ragle, 2002). They named this new data base, Combined DNA Index System (CODIS), the genetic equivalent to the Automated Fingerprint Identification System (AFIS). By 1994, CODIS was operational, but it wasn’t until 1999-2000 that most labs nationwide started relying on it as the official data base for sharing among agencies (Genge, 2002). Once most labs began testing the same thirteen STR points (1999-2000), CODIS could then be used to cross reference DNA Profiles from all over the United States; a practice that is widely used today. As of December 2004, CODIS contained 2,132,470 DNA profiles; and as of June 2009, over 7,137,468 offender profiles; and has assisted in more than 91,800 investigations (Federal Bureau of Investigation, n.d.).

Audersirk, T., Audersirk, G., & Byers, B. E. (2007). Biology: Life on Earth with Physiology (8th ed.). Upper Saddle River, NJ: Pearson-Prentice Hall.

Federal Bureau of Investigation - Laboratory Services. (n.d.). In Federal Bureau of Investigation Homepage. Retrieved August 04, 2009, from http://www.fbi.gov/hq/lab/codis/clickmap.htm

Genge, N. E. (2002). The Forensic Casebook The Science of Crime Scene Investigation. New York, NY: Ballantine Books.

Ragle, L. (2002). Crime scene from fingerprints to DNA testing, an astonishing inside look at the real world of c.s.i. New York, NY: Avon Books.

Trimm, H. H. (2005). Forensics the easy way. Hauppauge, NY: Barron's.